The significance of Xylella fastidiosa type I and type IV pili in biofilm structure, bacterial survival in biofilms and DNA se
نویسندگان
چکیده
Principal Investigator: CoPrincipal Investigator: Cooperator: Thomas J. Burr Harvey C. Hoch Steven Lindow Dept. of Plant Pathology Dept. of Plant Pathology Dept. of Plant and Microbial Biology Cornell University Cornell University University of California NYSAES NYSAES 111 Koshland Hall Geneva, New York 14456 Geneva, New York 14456 Berkeley CA 94720-3112 Phone: 315-787-2312 Phone: 315-787-2312 Phone: 510-642-4174 Email: [email protected] Email: [email protected] Email: [email protected]
منابع مشابه
Mutations in type I and type IV pilus biosynthetic genes affect twitching motility rates in Xylella fastidiosa.
Xylella fastidiosa possesses both type I and type IV pili at the same cell pole. By use of a microfluidic device, the speed of twitching movement by wild-type cells on a glass surface against the flow direction of media was measured as 0.86 (standard error [SE], 0.04) microm min(-1). A type I pilus mutant (fimA) moved six times faster (4.85 [SE, 0.27] microm min(-1)) and a pilY1 mutant moved th...
متن کاملCONTINUED ASSESSMENT OF XYLELLA FASTIDIOSA FIMBRIAL ADHESINS AS IMPORTANT VIRULENCE FACTORS IN PIERCE’S DISEASE: INFLUENCE OF XYLEM SAP Principal Investigator:
Specific biological characteristics of Xylella fastidiosa (Xf) Temecula were investigated in microfluidic flow chambers in vitro by examining the effect of xylem sap from Pierce’s disease (PD) susceptible V. vinifera and resistant V. smalliana grapevines on Xf cell growth, aggregation, biofilm formation, and motility. Growth of Xf was observed in both V. smalliana and V. vinifera xylem saps in ...
متن کاملUNDERSTANDING CONTROL OF XYLELLA FASTIDIOSA CELL AGGREGATION: IMPORTANCE IN COLONIZATION AND BIOFILM DEVELOPMENT IN GRAPEVINE AND SHARPSHOOTER FOREGUT Project Co-Leaders:
Our investigation focused on aggregation and ‘autoaggregation’of Xylella fastidiosa (Xf) cells. This study is particularly directed toward elucidating how Xf cells aggregate to cause PD symptoms. We have documented that both type IV and type I pili are important in the process. Furthermore, it was discerned that surface properties of WT cells change from being more hydrophobic to more hydrophil...
متن کاملTHE ROLES THAT DIFFERENT PILI CLASSES IN XYLELLA FASTIDIOSA PLAY IN COLONIZATION OF GRAPEVINES AND PIERCE’S DISEASE PATHOGENESIS Project Leaders:
Type I and type IV pili of Xylella fastidiosa play different roles in twitching motility, biofilm formation, and cell-cell aggregation. Thirty twitching mutants were generated with an EZ::TN transposome system and type IV pilus-associated genes were identified, including fimT, pilX, pilY1, pilO, and pilR. Mutations in all resulted in a twitch-minus phenotype except that pilY1 mutant was twitchi...
متن کاملCharacterization of regulatory pathways in Xylella fastidiosa: genes and phenotypes controlled by algU.
Many virulence genes in plant bacterial pathogens are coordinately regulated by "global" regulatory genes. Conducting DNA microarray analysis of bacterial mutants of such genes, compared with the wild type, can help to refine the list of genes that may contribute to virulence in bacterial pathogens. The regulatory gene algU, with roles in stress response and regulation of the biosynthesis of th...
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